Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)
CAT#: TAB-457CQ
Recombinant monoclonal antibody to TNFRSF9. This antibody is a human monoclonal antibody that specifically binds to the CD137 receptor (also called 4-1BB).
Tested Data
Gene Expression
WB
Figure 1 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in WB
Western blot analysis of TAB-457CQ was performed with Recombinant Human TNFRSF9 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-457CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in ELISA
ELISA analysis of TAB-457CQ was performed by coating with Recombinant Human TNFRSF9 Protein. Then blocked with BSA, and incubated with Anti-Human TNFRSF9 Antibody (TAB-457CQ). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 3 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in SDS-PAGE
SDS-PAGE analysis of TAB-457CQ-LowE in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
WB
Figure 4 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in WB
Western blot analysis of TAB-457CQ-LowE was performed by loading human TNFRSF9 protein, His tag.
ELISA
Figure 5 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in ELISA
ELISA analysis of TAB-457CQ-LowE was performed by coating with human TNFRSF9 protein (His tag). Then blocked with BSA and incubated with TAB-457CQ-LowE. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71425/1522_F4_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049249-TNFRSF9
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG2, λ
- Specificity
- Human receptor 4-1BB
- Species Reactivity
- Human
- Applications
- ELISA, IHC, FC, IP, IF, FuncS
- Conjugate
- Unconjugated
- Related Disease
- B cell lymphoma, Non-Hodgkin's lymphoma, Solid tumours
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The TNFRSF9 antibody has been reported in applications of SPR, Activ, IF.
Target
- Alternative Names
- TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; 4-1BB; CD137; CDw137; tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63
- Gene ID
- 3604
- UniProt ID
- Q07011
REVIEWS AND Q&AS
CITATIONS
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Versatile and Reliable
We've used this antibody across various assays from flow cytometry to functional studies. It consistently delivers high-quality data that form the backbone of our immune response investigations, proving its versatility.
Boosts Cancer Research
Utilized extensively within our cancer immunotherapy projects, this antibody helps unravel TNFRSF9's role in enhancing anti-tumor immunity, offering promising directions for developing new therapeutic strategies.
Q&As
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What applications does the Anti-Human TNFRSF9 Recombinant Antibody support?
A: This antibody is suitable for a variety of research applications including ELISA, flow cytometry (FC), immunohistochemistry (IHC), immunoprecipitation (IP), and functional assays. These uses enable detailed studies of immune response and receptor signaling pathways.
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What role does TNFRSF9 play in disease research?
A: TNFRSF9, also known as 4-1BB, is significant in immune checkpoint regulation. It is involved in co-stimulation and survival signaling of T cells and has implications in cancer immunotherapy and autoimmune disease research.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-457CQ, RRID: AB_3111955)
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Biosimilar Overview
Please refer to Utomilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Utomilumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TNFRSF9"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1443z | Mouse Anti-TNFRSF9 Recombinant Antibody (clone 29H11) | ELISA, FC, IP, FuncS | Mouse IgG1 |
| HPAB-0053-FY | Human Anti-TNFRSF9 Recombinant Antibody (HPAB-0053-FY) | ELISA | Human IgG2 |
| HPAB-0809LY | Human Anti-TNFRSF9 Recombinant Antibody (HPAB-0809LY) | FC, FuncS | Human IgG1 |
| HPAB-0810LY | Human Anti-TNFRSF9 Recombinant Antibody (HPAB-0810LY) | FC, FuncS | Human IgG1 |
| HPAB-0811LY | Human Anti-TNFRSF9 Recombinant Antibody (HPAB-0811LY) | FC, FuncS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-179 | Anti-Human 4-1BB Recombinant Antibody (TAB-179) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG4 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G063S | Anti-TNFRSF9 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-064LC | Recombinant Anti-Human TNFRSF9 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-064LC-1 | Recombinant Anti-Human TNFRSF9 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0611CLV | Human Anti-TNFRSF9 Recombinant Antibody (TAB-0611CLV) | ELISA, FC | Humanized IgG1 |
| TAB-0612CLV | Human Anti-TNFRSF9 Recombinant Antibody (TAB-0612CLV) | ELISA, FC | Humanized IgG4 |
| TAB-0611CLV-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (TAB-0611CLV-F(E)) | ELISA, FC | Humanized Fab |
| TAB-0611CLV-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (TAB-0611CLV-S(P)) | ELISA, FC | Humanized scFv |
| TAB-0612CLV-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (TAB-0612CLV-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0613CLV | Human Anti-TNFRSF9 Recombinant Antibody (TAB-0613CLV) | ELISA | Chimeric (mouse/human) IgG1 |
| TAB-0614CLV | Human Anti-TNFRSF9 Recombinant Antibody (TAB-0614CLV) | ELISA | Chimeric (mouse/human) IgG1 |
| TAB-0613CLV-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (TAB-0613CLV-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| TAB-0613CLV-S(P) | Mouse Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (TAB-0613CLV-S(P)) | ELISA | Mouse scFv |
| TAB-0614CLV-S(P) | Mouse Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (TAB-0614CLV-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2167CQ | Rat Anti-Tnfrsf9 Recombinant Antibody (clone CBL332) | Block, WB | Rat IgG2b |
| NEUT-2168CQ | Hamster Anti-Tnfrsf9 Recombinant Antibody (clone 17B5) | FC, Block | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2169CQ | Rat Anti-Tnfrsf9 Recombinant Antibody (NEUT-2169CQ) | Neut, WB | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0809LY-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (HPAB-0809LY-S(P)) | FC, FuncS | Human scfv |
| HPAB-0810LY-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (HPAB-0810LY-S(P)) | FC, FuncS | Human scfv |
| HPAB-0811LY-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (HPAB-0811LY-S(P)) | FC, FuncS | Human scfv |
| HPAB-0812LY-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (HPAB-0812LY-S(P)) | FC, FuncS | Human scfv |
| HPAB-0813LY-S(P) | Human Anti-TNFRSF9 Recombinant Antibody; scFv Fragment (HPAB-0813LY-S(P)) | FC, FuncS | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0809LY-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (HPAB-0809LY-F(E)) | FC, FuncS | Human Fab |
| HPAB-0810LY-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (HPAB-0810LY-F(E)) | FC, FuncS | Human Fab |
| HPAB-0811LY-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (HPAB-0811LY-F(E)) | FC, FuncS | Human Fab |
| HPAB-0812LY-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (HPAB-0812LY-F(E)) | FC, FuncS | Human Fab |
| HPAB-0813LY-F(E) | Human Anti-TNFRSF9 Recombinant Antibody; Fab Fragment (HPAB-0813LY-F(E)) | FC, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-457CQ | Afuco™ Anti-TNFRSF9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-457CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-179 | Afuco™ Anti-TNFRSF9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-179) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY51 | Rat Anti-Tnfrsf9 Agonistic Antibody (VS-0724-XY51) | Agonistic assays | Rat IgG2a |
| VS-0724-XY52 | Rabbit Anti-TNFRSF9 Agonistic Antibody (VS-0724-XY52) | FC, Agonistic assays, CyTOF® | Rabbit IgG |
| VS-0724-XY53 | Human Anti-TNFRSF9 Agonistic Antibody (VS-0724-XY53) | ELISA, Agonistic assays | Human IgG1 |
| VS-0724-XY54 | Rabbit Anti-TNFRSF9 Agonistic Antibody (clone RbBBK4) | ELISA, FC, Agonistic assays | Rabbit IgG, kappa |
| VS-0724-XY55 | Mouse Anti-TNFRSF9 Agonistic Antibody (VS-0724-XY55) | ELISA, FC, Agonistic assays | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY76 | Mouse Anti-NHP TNFRSF9 Recombinant Antibody (VS-1024-XY76) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY224 | CytoStream™ Rat Anti-TNFRSF9 Recombinant Antibody (VS-0225-XY224) | FC | Rat IgG1, kappa |
| VS-0225-XY225 | CytoStream™ Mouse Anti-TNFRSF9 Recombinant Antibody (VS-0225-XY225) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY100 | Human Anti-TNFRSF9 (clone 39E3) scFv-Fc Chimera | FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC223 | Recombinant Anti-TNFRSF9 Vesicular Antibody, EV Displayed (VS-0425-YC223) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7386 | Anti-TNFRSF9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC236 | Recombinant Anti-TNFRSF9 Biparatopic Antibody, Tandem scFv | ELISA, IF, IP | Tandem scFv |
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